FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13683416
Sequences flagged as poor quality0
Sequence length54-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGTA164850.011.281704120
AGAGTGT191350.010.471766120
TAGATCT106500.010.421352120
AGTGTAG148750.010.324879120
TAGGGAA339200.010.081403120
AAGAGTG225650.010.023236120
TGTAGGG409850.09.207202120
GTGTAGG444800.09.1581335120
GTGTAGA166200.09.132538120
GTAGGGA360350.09.09013120
CGTGTAG474700.08.821413120
GTAGATC185700.08.787371120
GTCGTGT544450.08.6719885120
TCGTGTA509600.08.511593120
CGTCGTG628050.07.890196120
AAAGAGT285000.07.8306904120
AGCGTCG714400.07.801466120
GGGAAAG380300.07.4143567120
AAGAGCG876050.07.402847120
AGGGAAA422400.07.3997283120
Produced by FastQC (version 0.11.5)