FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13699216
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCGC174350.010.155033120
TAGATCT378900.010.121795120
AGTGTAG532650.09.70158120
GCGTCGG70450.09.626728120
GTGGTCG193700.09.388458120
GAGTGTA579500.09.362596120
GGTGGTC216050.09.306188120
ATCTCGG324750.09.277604120
GATCTCG360150.09.248917120
GTGTAGA519800.09.190901120
AGAGTGT631950.09.155363120
AGATCTC371250.09.117881120
CTCGGTG274250.09.1039095120
TCTCGGT291150.08.925907120
TGTAGAT562450.08.579331120
GGTCGCC176750.08.285366120
CGGTGGT263550.08.221016120
TCGGTGG263700.08.190857118-119
AAGAGTG704150.08.156955120
GTAGATC540000.08.0913105120
Produced by FastQC (version 0.11.5)