FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13699216
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC144500.079.62723120
GAGCACA232100.049.65946120
AGAGCAC324500.036.537464120
AAGAGCA508900.023.674723120
GAAGAGC571200.020.94483118-119
GGAAGAG731750.017.467245120
TCGGAAG849800.014.803883118-119
CGGAAGA859400.014.611301120
GATCGGA1005850.012.58905120
ATCGGAA995150.012.584946120
AGATCGG1098550.011.460772118-119
TAGATCG271200.011.356039120
CTAGATC62700.010.413404116-117
TTAGATC97950.09.648554120
GAGATCG422750.08.985949116-117
AAGATCG402150.08.627835120
GCTAGAT27150.08.502043116-117
CAGATCG368650.08.148955118-119
TCTAGAT36850.07.8912506120
TAAGATC167650.07.217901110-111
Produced by FastQC (version 0.11.5)