FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10970826
Sequences flagged as poor quality0
Sequence length50-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC133450.071.0376120
GAGCACA205200.044.014492120
AGAGCAC275550.035.16778120
AAGAGCA415550.022.829401120
GAAGAGC464700.020.823973120
GGAAGAG569050.017.2321120
TCGGAAG672650.015.08283120
CGGAAGA675500.014.747765120
ATCGGAA796700.013.12645120
GATCGGA814200.012.710938118-119
CTAGATC51550.011.592611112-113
AGATCGG904800.011.321825118-119
CACACGT36150.011.083198120
TAGATCG202350.010.739108120
ACACGTC41400.010.169807120
TTAGATC69200.09.666209116-117
CACGTCT38300.08.865284120
AAGATCG363250.08.76776120
CCTAGAT18100.08.629203120
GAGATCG359050.08.586714116-117
Produced by FastQC (version 0.11.5)