FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11567589
Sequences flagged as poor quality0
Sequence length57-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCTC381800.09.181995120
GTGGTCG220950.09.171499120
TAGATCT388600.08.882056120
TGGTCGC199600.08.555832120
ATCTCGG343650.08.451528120
GCGTCGG85100.08.408564120
TCTCGGT313650.08.397176120
CTCGGTG300550.08.363036120
GAGTGTA562100.08.215842120
GGTCGCC211250.08.083995120
AGTGTAG532550.08.016825120
AGAGTGT609150.07.92676120
GGTGGTC239500.07.853257118-119
TCGGTGG281350.07.8437467120
CGGTGGT278050.07.785455120
GTGTAGA516550.07.7645793120
TGTAGAT548450.07.7515187120
GATCTCG383250.07.719468120
GTAGATC567050.07.5820937120
AAGAGTG669550.07.4721355120
Produced by FastQC (version 0.11.5)