FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11567589
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC125250.071.21953120
GAGCACA195150.043.54271120
AGAGCAC255750.034.2824118-119
AAGAGCA406150.021.995472120
GAAGAGC457300.019.434086120
GGAAGAG550000.017.528133120
CGGAAGA647150.014.855972120
TCGGAAG658950.014.634659118-119
ATCGGAA775450.012.721814120
GATCGGA801800.012.080766116-117
CTAGATC82200.011.977261120
AGATCGG869800.011.136728120
TAGATCG321900.010.981858120
TTAGATC116250.09.261269118-119
CAGATCG458350.08.655808116-117
GCTAGAT30650.08.48618118-119
ATAGATC143000.07.9663224116-117
GAGATCG249400.07.3388224120
CACGTCT42350.07.333098120
TCTAGAT40750.07.2716103118-119
Produced by FastQC (version 0.11.5)