FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11964821
Sequences flagged as poor quality0
Sequence length67-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA639350.010.369191120
GGTCGCC447300.010.126731120
GCGTCGG64500.09.580733120
CGTATCA302250.09.230132120
CGCCGTA369850.08.970585120
TGGTCGC464100.08.900595118-119
GCCGTAT366650.08.868881120
GTGGTCG505800.08.860598120
GGTGGTC552400.08.732199120
GTCGCCG438500.08.647065120
TCGCCGT409200.08.474488120
TCATTAA267300.08.237366120
CTCGGTG671300.08.186534120
CCGTATC363550.07.9543395120
TCGGTGG644900.07.8146253120
TCTCGGT718000.07.7125597120
CGGTGGT618950.07.628522120
GATCTCG828950.07.4764123120
ATCTCGG772750.07.468911120
TAGATCT901650.07.4325256120
Produced by FastQC (version 0.11.5)