FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13116139
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC130450.063.23082120
GAGCACA205800.041.348335120
AGAGCAC276400.033.407516120
AAGAGCA438150.020.486828118-119
GAAGAGC468500.019.048231118-119
GGAAGAG575750.015.777242120
CGGAAGA662100.014.360243120
TCGGAAG655800.014.219939118-119
ATCGGAA779100.012.11608118-119
GATCGGA775550.012.045477116-117
AGATCGG863850.010.965317116-117
TAGATCG208300.010.557631120
CTAGATC50150.010.475323116-117
CACGTCT42250.010.348416120
CACACGT37300.09.447367120
TTAGATC95350.09.0339775120
AAGATCG367350.08.482733116-117
ACACGTC45500.08.175029120
GAGATCG345050.08.170347114-115
CCTAGAT20650.07.2685294114-115
Produced by FastQC (version 0.11.5)