FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831507

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831507
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11402518
Sequences flagged as poor quality0
Sequence length50-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC130800.074.0177120
GAGCACA208350.048.528347120
AGAGCAC273500.036.132896118-119
AAGAGCA417250.024.066216118-119
GAAGAGC454900.021.680172116-117
GGAAGAG552250.018.421383120
CGGAAGA643950.015.518522118-119
TCGGAAG662250.015.110611118-119
ATCGGAA784850.013.341396120
GATCGGA794250.012.735413116-117
AGATCGG882400.011.576111118-119
TAGATCG228700.011.08367116-117
CACGTCT40750.09.856813120
CACACGT33750.09.644068118-119
CTAGATC57100.09.581975112-113
GAGATCG336100.08.993979118-119
CAGATCG317350.08.969506114-115
ACACGTC41350.08.876393118-119
TTAGATC92500.08.834954112-113
ATAGATC120200.08.815016120
Produced by FastQC (version 0.11.5)