FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14575767
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC209800.089.11647120
GAGCACA341600.053.44856120
AGAGCAC463500.040.626644120
AAGAGCA705950.026.499369118-119
GAAGAGC774150.023.9248118-119
GGAAGAG951400.019.619238116-117
CGGAAGA1115300.016.662758116-117
TCGGAAG1139950.016.289516114-115
CACACGT61350.015.631497120
ATCGGAA1361000.014.256333120
CACGTCT65950.014.203037120
GATCGGA1399550.013.536573118-119
AGATCGG1530550.012.433631112-113
ACACGTC67350.012.141368118-119
TAGATCG454750.011.679978118-119
ACGTCTG84400.010.833982120
GCACACG77150.010.406707120
GAGATCG634600.010.261471110-111
CTAGATC70000.010.247899108-109
CAGATCG619500.010.091443116-117
Produced by FastQC (version 0.11.5)