FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12772691
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC173900.092.388596120
GAGCACA288800.055.245934120
AGAGCAC383500.041.873337120
AAGAGCA560900.028.821177118-119
GAAGAGC649200.025.238022120
GGAAGAG771200.021.044563116-117
CGGAAGA902600.017.932285118-119
TCGGAAG936950.017.270817114-115
ATCGGAA1079100.015.66997120
GATCGGA1116050.014.766333118-119
AGATCGG1229500.013.339259114-115
TAGATCG315150.012.8460865116-117
CACGTCT50200.012.358264120
CTAGATC81400.012.311691110-111
CACACGT46350.011.840387120
TTAGATC92850.011.393051116-117
CCTAGAT29550.011.170038108-109
GAGATCG422950.010.700273112-113
CAGATCG427600.010.630413114-115
AAGATCG375100.010.58093116-117
Produced by FastQC (version 0.11.5)