FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12653013
Sequences flagged as poor quality0
Sequence length50-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC111300.064.58008120
GAGCACA170300.042.81431120
AGAGCAC230800.031.899666120
AAGAGCA360700.021.39804120
GAAGAGC416150.018.166922118-119
GGAAGAG533150.014.683029120
TCGGAAG596450.013.938267120
GATCGGA668300.012.391989118-119
ATCGGAA681750.012.1658535120
AGATCGG746250.011.608495120
CGGAAGA686400.011.45193120
TAGATCG185500.010.567649120
CTAGATC43050.010.144558118-119
GAGATCG296450.09.10048120
CAGATCG281500.08.825361120
TTAGATC64850.08.77961118-119
AAGATCG221000.08.592477118-119
TGAGATC106000.08.056525120
CCTAGAT17100.07.945164120
ACTAGAT17752.5826012E-87.289728120
Produced by FastQC (version 0.11.5)