FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13095946
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC143700.069.56602120
GAGCACA218100.049.08931120
AGAGCAC292150.035.395725118-119
AAGAGCA472650.023.19013120
GAAGAGC514700.020.233858116-117
GGAAGAG627050.016.949673118-119
CGGAAGA758050.014.247236120
TCGGAAG758750.014.066425120
ATCGGAA879150.012.370604118-119
GATCGGA868600.012.328465118-119
AGATCGG967500.011.335927120
TAGATCG250600.010.794298120
CTAGATC61900.010.059732120
CACACGT45200.09.924999120
TTAGATC100550.09.506493118-119
GAGATCG341950.08.952993116-117
AAGATCG341550.08.821702120
CACGTCT49000.08.745366120
CCTAGAT21400.08.433646114-115
TGAGATC131200.08.216487120
Produced by FastQC (version 0.11.5)