FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14731590
Sequences flagged as poor quality0
Sequence length57-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGGT21250.021.6101120
TCTAGGG15400.021.252089116-117
GTCTAGG14950.021.006468114-115
AGTCTAG22100.019.888416120
CGTCTAG23650.019.139723120
CTAGGTG12750.017.241781114-115
CTAGGGA11750.016.201908108-109
CTAGGGC6350.014.653671116-117
CGTAGTG54150.013.447519120
CTAGGGT5100.013.212084116-117
ATCTAGG19350.012.37362118-119
CGAGTAG37000.011.967726114-115
GATCTAG20950.011.741731118-119
AGTAGGG28300.011.609397108-109
GAGTAGG40500.011.487755116-117
AAAAAAA1168300.011.162968120
GTCTAGA29200.010.862576114-115
TCGAGTA57100.010.684753120
CTCAGTG38900.010.624503120
CGTATCA445750.010.463957120
Produced by FastQC (version 0.11.5)