FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15177270
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC211400.093.16673120
GAGCACA353850.056.784676120
AGAGCAC469050.042.526546118-119
AAGAGCA702950.028.695154120
GAAGAGC814900.024.617409116-117
CCGATCT89550.021.021014
GGAAGAG965050.020.88327118-119
TCCGATC96400.019.86693
CGATCTA24350.018.8226325
CGGAAGA1141350.017.661877114-115
TCGGAAG1176750.017.12366116-117
ATCGGAA1365000.014.812257112-113
GATCGGA1394600.014.532762114-115
CTTCCGA134600.014.3349921
AGATCGG1523800.013.276757110-111
TAGATCG383400.012.379045112-113
CTAGATC92650.012.018662112-113
CACGTCT66050.011.455218120
CAGATCG582050.010.803084112-113
TTAGATC146700.010.774312114-115
Produced by FastQC (version 0.11.5)