FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13466599
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC194250.078.099266120
GAGCACA310450.048.82011120
AGAGCAC412100.037.87477120
AAGAGCA622050.025.665796120
GAAGAGC700150.023.281805120
GGAAGAG840400.019.344378120
CGGAAGA986900.016.864313120
TCGGAAG1016500.016.100782118-119
ATCGGAA1206600.013.697224116-117
CACGTCT66150.013.555363120
GATCGGA1235050.013.390555116-117
AGATCGG1355300.012.205661114-115
TAGATCG324400.011.798162120
CACACGT57300.011.704939120
ACACGTC66850.011.614118118-119
CTAGATC92900.010.829483116-117
GAGATCG505050.09.794516112-113
TTAGATC121900.09.748688114-115
ACGTCTG80450.09.605407120
GCACACG84900.09.359545120
Produced by FastQC (version 0.11.5)