FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13585727
Sequences flagged as poor quality0
Sequence length60-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG83600.09.831314120
GCCTAAC25450.09.07646100-101
TAGATCT460400.08.991063120
GTGGTCG242500.08.955587120
ATCTCGG400050.08.637846120
CCCCCGT28150.08.63134942-43
GTGTAGA588050.08.610442120
AGATCTC448000.08.4766245120
TCGGTGG314050.08.424362120
TATCCCC64250.08.4036245
GATCTCG428200.08.378221120
AGTGTAG607100.08.360022120
GGTGGTC271800.08.255044120
GTAGATC580650.08.141586120
AGAGTGT697500.08.016208120
GAGTGTA643950.07.9934154120
TATACCC62600.07.95428289
CTCGGTG346300.07.8650336120
TGGTCGC218400.07.801228120
TCCCCAT72700.07.7569457
Produced by FastQC (version 0.11.5)