FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13585727
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC140900.067.6746120
GAGCACA215600.041.211563120
AGAGCAC295750.033.573536120
AAGAGCA469200.020.519457118-119
GAAGAGC512700.018.854477120
GGAAGAG604400.015.886464116-117
TCGGAAG692150.014.563917120
CGGAAGA687350.014.17948116-117
ATCGGAA808850.012.357103120
GATCGGA818300.012.302679120
AGATCGG905900.011.070026118-119
GCCTAAC24550.010.230985100-101
TAGATCG225350.010.171538114-115
CACACGT30950.09.123844120
AAGATCG396050.08.78052116-117
CCAGGGG29600.08.766096112-113
CCTAACT26800.08.7458515102-103
CTAGATC71000.08.509411120
TATCCCC66300.08.4261455
TATACCC58250.08.2918869
Produced by FastQC (version 0.11.5)