FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14945394
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC158400.067.480194120
GAGCACA241450.043.94712120
AGAGCAC315900.033.89782120
AAGAGCA500750.022.084120
GAAGAGC555850.020.198252120
GGAAGAG701100.017.133076120
TCGGAAG836200.014.434792120
CGGAAGA837250.014.408942120
GATCGGA995150.012.774439120
ATCGGAA992150.012.623485120
AGATCGG1095000.011.509006118-119
TAGATCG270400.011.29766120
CTAGATC69450.010.366329120
GAGATCG412950.09.345312120
TTAGATC117300.09.068181120
AAGATCG433400.08.84477114-115
ACACGTC50700.08.187406120
CAGATCG334250.07.761894118-119
GCTAGAT29900.07.4838014112-113
TGAGATC173600.07.360302118-119
Produced by FastQC (version 0.11.5)