FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14407285
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC151000.063.538536120
GAGCACA224700.041.770123120
AGAGCAC299550.032.507805120
AAGAGCA466350.022.082664120
GAAGAGC507600.020.236757120
GGAAGAG635750.016.567665120
TCGGAAG743000.014.123535120
CGGAAGA749450.014.02802118-119
ATCGGAA885750.012.252068120
GATCGGA885700.012.068784120
AGATCGG962350.011.283616120
TAGATCG207650.011.205794120
CTAGATC52900.09.2408495120
AAGATCG456000.08.76194118-119
TTAGATC94500.08.345548114-115
GAGATCG338050.07.9145904112-113
CAGATCG321050.07.1664658116-117
CAAGATC269550.06.673844120
TAAGATC183500.06.642122114-115
GCTAGAT25550.06.249949114-115
Produced by FastQC (version 0.11.5)