FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14513613
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC163250.077.13074120
GAGCACA262750.049.335587120
AGAGCAC356750.036.48756120
AAGAGCA556450.024.61828120
GAAGAGC631300.021.182045118-119
GGAAGAG771750.017.44417120
TCGGAAG925600.014.705133120
CGGAAGA933650.014.592807120
ATCGGAA1087850.012.698091120
GATCGGA1104950.012.471026120
AGATCGG1211300.011.643634120
TAGATCG271050.010.959862120
CTAGATC58300.010.401309110-111
TTAGATC111450.09.190013118-119
GAGATCG522600.09.154277114-115
AAGATCG394950.08.803597118-119
CAGATCG402950.07.959914118-119
TGAGATC209200.07.7300916114-115
TAAGATC168200.07.4714594118-119
GCTAGAT24600.07.410209120
Produced by FastQC (version 0.11.5)