FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13719953
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC123550.062.646084120
GAGCACA203000.039.14191120
AGAGCAC256200.031.21498120
AAGAGCA401400.020.676804120
GAAGAGC459000.017.889051118-119
GGAAGAG555800.015.08332120
TCGGAAG640950.013.832355120
CGGAAGA638800.013.455896120
ATCGGAA740750.011.8992605120
GATCGGA759600.011.688672120
AGATCGG845300.010.770022120
TAGATCG193250.09.975227116-117
CTAGATC55100.08.673661116-117
TTAGATC78600.08.406447116-117
CAGATCG330950.07.82116118-119
GAGATCG408500.07.1765237118-119
AAGATCG310650.06.958874120
CCTAGAT23200.06.6379437110-111
CACGTCT51100.05.791726120
CCCTAGA9605.6200737E-55.681672108-109
Produced by FastQC (version 0.11.5)