FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17577331
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC196800.071.56424120
GAGCACA316650.047.034058120
AGAGCAC423150.034.356487120
AAGAGCA656300.022.722958120
GAAGAGC732300.019.933332120
GGAAGAG942050.016.452211120
TCGGAAG1081650.014.401869120
CGGAAGA1143200.013.540119120
ATCGGAA1289400.012.719433120
GATCGGA1318150.012.1844118-119
CACACGT66550.011.669207120
AGATCGG1439500.011.232549118-119
CACGTCT76750.010.804372120
ACACGTC75350.010.393722120
TAGATCG270850.010.34397116-117
AAGATCG642550.09.463949120
GAGATCG463950.09.404804120
CTAGATC78850.09.372337116-117
CAGATCG477650.08.790595120
GCACACG91300.08.5779505120
Produced by FastQC (version 0.11.5)