FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13107451
Sequences flagged as poor quality0
Sequence length50-126
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC221150.0145.09993120
GAGCACA372350.080.568405120
AGAGCAC492900.062.848606118-119
AAGAGCA751900.040.462852120
GAAGAGC825550.037.34661116-117
GGAAGAG978600.030.10768118-119
CCGATCT72750.028.0199014
CGGAAGA1138400.027.221483114-115
TCCGATC78500.026.218993
TCGGAAG1198150.024.45932116-117
CGATCTA19500.023.2864235
CACACGT56550.022.51296120
ATCGGAA1394050.022.454647112-113
ACACGTC59700.021.87189120
CTTCCGA99150.021.8397271
GATCGGA1439150.020.49917114-115
CACGTCT58600.020.474691118-119
AGATCGG1547550.020.174265110-111
ACGTCTG69100.018.73908120
CGTCTGA79150.018.146898120
Produced by FastQC (version 0.11.5)