FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11671615
Sequences flagged as poor quality0
Sequence length51-126
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT30150.030.8443834
TCCGATC38850.024.8637473
CCCTACG2653.405148E-822.6488341
CCCCACG2209.565126E-519.0970861
CGGTGGG5052.1827873E-1117.821056120
CGATCTA9150.017.704125
CCCTTAG8150.016.201561
AAAAAAA4337750.014.968441120
CGATCTC26150.014.6838515
CTTCCGA74500.014.5818981
CCCTCAC6608.185452E-1114.5501611
CCCCGCA4801.1207012E-613.754451
CCCCTTA4951.5844762E-613.3376471
CCCTACA5905.9169906E-813.2246161
CCCTTCA16550.012.6929261
CCTCTAG10700.012.3404411
CCCTCGA6351.5443584E-712.2874381
CCCTACT3955.793005E-412.155831
CCCCTGT4952.3184239E-512.1251341
CCCTGCC7457.747076E-912.0844461
Produced by FastQC (version 0.11.5)