FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11671615
Sequences flagged as poor quality0
Sequence length50-126
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC163350.0202.04576120
GAGCACA289150.0107.560555120
AGAGCAC389200.082.35523118-119
AAGAGCA594200.050.7284118-119
GAAGAGC642900.049.83772116-117
GGAAGAG777450.038.755856116-117
CGGAAGA901650.035.649124114-115
CACACGT30800.032.217537120
TCGGAAG962000.031.257736114-115
CACGTCT35850.029.195896120
CCGATCT33100.028.9987244
ATCGGAA1119250.028.950224112-113
ACACGTC32850.028.138033118-119
GATCGGA1125800.026.898022112-113
AGATCGG1195050.026.792442110-111
ACGTCTG43550.025.28233120
TCCGATC40000.024.5760693
GCACACG32600.023.350203118-119
TAGATCG258850.022.71671110-111
GAGATCG500400.022.358692112-113
Produced by FastQC (version 0.11.5)