FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18124735
Sequences flagged as poor quality0
Sequence length52-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG140250.010.608739120
TGGTCGC397150.09.4717120
GGTGGTC488150.09.205418120
CGTATCA246600.08.807051120
ATCTCGG708200.08.801863120
AGATCTC799350.08.631292120
GTGGTCG437200.08.576594120
TAGATCT830950.08.562977120
TCGGTGG577150.08.49275120
GAGTGTA1176950.08.431259120
GATCTCG791400.08.399602120
CTCGGTG643100.08.218875120
TCTCGGT652750.08.207664120
AGTGTAG1102450.08.152069120
GTGTAGA1066850.08.131673120
GGTCGCC395000.07.974018120
AGAGTGT1275750.07.919386120
AAGAGTG1389950.07.8082623120
GTAGATC1143850.07.794078120
CGGTGGT561550.07.735086120
Produced by FastQC (version 0.11.5)