FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13625390
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC112400.046.72897120
GAGCACA157150.036.536083120
AGAGCAC210400.028.466715120
GAAGAGC380500.017.64539120
AAGAGCA372400.016.848312120
GGAAGAG470200.013.989382120
TCGGAAG542550.013.881948120
GATCGGA653200.012.355346120
AGATCGG704150.011.980324120
CGGAAGA580750.011.94498120
CTAGATC44250.011.757708120
TAGATCG176050.011.363788120
ATCGGAA683900.011.139589120
TTAGATC82950.09.184285120
CACACGT33750.08.625429120
GAGATCG275600.08.450166120
AAGATCG331300.08.263375120
GCTAGAT19250.07.8830085118-119
CCTAGAT16400.07.7422404118-119
TCTAGAT27150.06.958035118-119
Produced by FastQC (version 0.11.5)