FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14575159
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC186700.075.348564120
GAGCACA284400.048.47429120
AGAGCAC385450.036.78149120
AAGAGCA584850.024.173578118-119
GAAGAGC675200.021.332924120
GGAAGAG830500.017.299946118-119
CGGAAGA1006100.014.773864120
TCGGAAG996850.014.656125120
ATCGGAA1149900.012.962151120
GATCGGA1165450.012.610334118-119
AGATCGG1279750.011.486682116-117
TTAGATC96900.010.982096120
TAGATCG260200.010.71145114-115
CTAGATC70050.010.472858112-113
GCTAGAT27250.09.686879112-113
AAGATCG514800.09.402165120
GAGATCG441500.08.882904112-113
CACGTCT54650.08.668345120
CAGATCG431300.08.61618116-117
CCTAGAT25150.08.451119108-109
Produced by FastQC (version 0.11.5)