FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10880965
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC128550.069.90553120
GAGCACA212750.041.278362120
AGAGCAC273400.033.110046120
AAGAGCA433400.021.662514120
GAAGAGC465150.019.73817118-119
GGAAGAG567300.017.002756120
TCGGAAG651600.015.632742120
CGGAAGA614700.015.616579120
ATCGGAA773100.013.329415120
GATCGGA792850.013.051939118-119
AGATCGG879250.011.836871118-119
TAGATCG223000.011.782832116-117
CACACGT36200.010.290693118-119
CACGTCT36600.010.267898120
ACACGTC38600.010.248298120
CAGATCG336150.010.199009120
CTAGATC56250.09.788909114-115
GAGATCG311500.09.757322120
AAGATCG242300.09.714099120
TTAGATC82300.08.69273116-117
Produced by FastQC (version 0.11.5)