FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14988796
Sequences flagged as poor quality0
Sequence length55-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG69600.014.594163120
AAAAAAA2614300.014.559812120
TCTCGGG31750.012.872619120
GTATCAT1080500.09.183834120
CGTATCA1115050.09.034027120
CCGTATC1225650.09.01814120
GCCGTAT1297700.08.749019120
TCATTAA874650.08.610304120
TATCATT1097650.08.569434120
CGCCGTA1376550.08.448715120
GTCGCCG1595900.08.424847120
CATTAAA837950.08.34904120
TCGCCGT1499550.08.324866120
ATCATTA977400.08.147892120
TGGTCGC1794550.08.088411120
GGTCGCC1675250.07.828469120
CGGTGGG30050.07.800489120
GTGGTCG1916850.07.7730255120
GGTGGTC2048350.07.52049120
ATTAAAA955450.07.448099120
Produced by FastQC (version 0.11.5)