FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13856000
Sequences flagged as poor quality0
Sequence length53-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG110900.08.439855120
TAGATCT346850.08.009053120
GAGTGTA479950.07.475618120
AGATCTC341600.07.4471464120
TCTCGGT304850.07.439564120
AGTGTAG443600.07.411924120
AAGAGTG565550.07.076145120
CTCGGTG299750.07.04572120
GTAGATC471850.06.980882120
ATCTCGG339950.06.953814120
AGAGTGT513000.06.923827120
GTGTAGA451150.06.8959484118-119
GTAGGGA799250.06.868792120
CGGTGGT274300.06.802624120
TGTAGAT491450.06.8001385120
TGGTCGC214700.06.706888120
TGTAGGG844650.06.4782834120
GTCTAGG5100.00418164266.47055492-93
TCGGTGG280250.06.4655166120
TAGGGAA764450.06.3259807120
Produced by FastQC (version 0.11.5)