FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13856000
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC111900.066.3651120
GAGCACA172100.042.14903120
AGAGCAC242900.031.545969120
AAGAGCA388250.019.571568118-119
GAAGAGC451800.017.610104120
GGAAGAG547450.014.778224120
TCGGAAG616350.014.089696120
CGGAAGA638450.012.91188120
GATCGGA733150.012.115028120
ATCGGAA735100.012.0742035120
AGATCGG818800.011.346849120
TAGATCG233150.011.2837105120
CTAGATC57500.010.383264118-119
TTAGATC92300.08.30175118-119
AAGATCG330750.07.432775120
CAGATCG332550.07.315738118-119
GAGATCG347150.07.063216116-117
CCTAGAT23550.06.9139695114-115
ATAGATC122400.06.4167314118-119
TCTAGAT32450.06.3952684118-119
Produced by FastQC (version 0.11.5)