FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12844921
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC131800.075.07208120
GAGCACA207800.045.58661120
AGAGCAC281550.034.618195118-119
AAGAGCA440800.023.134138120
GAAGAGC489950.020.30248120
GGAAGAG596500.017.581526120
TCGGAAG709400.015.266328120
CGGAAGA707550.014.655229118-119
ATCGGAA835800.012.918157120
GATCGGA850250.012.892306120
AGATCGG948100.011.499763118-119
TAGATCG216100.011.12659120
CTAGATC55150.011.108652120
AAGATCG416350.09.082671116-117
TTAGATC98800.08.934531120
GAGATCG331300.08.232896114-115
CACACGT32900.08.009179120
ACACGTC41050.07.7028604120
ATAGATC98200.07.580378120
TAAGATC158450.07.527023108-109
Produced by FastQC (version 0.11.5)