FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16801830
Sequences flagged as poor quality0
Sequence length50-126
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC316750.0114.18216120
GAGCACA522900.069.56418120
AGAGCAC714300.050.769245118-119
AAGAGCA1067300.033.889412118-119
GAAGAGC1163700.031.021765116-117
GGAAGAG1392900.025.72083116-117
CACGTCT94200.025.582657120
ACACGTC94000.025.53655120
CACACGT91150.023.692307118-119
CGGAAGA1577450.022.70894114-115
TCGGAAG1691700.021.016401114-115
GCACACG99100.020.126362118-119
ACGTCTG116550.018.89296120
CGTCTGA127750.018.568174120
ATCGGAA1980250.018.375586112-113
GATCGGA2043650.017.683233112-113
AGATCGG2240250.016.400314110-111
TAGATCG602400.014.778037110-111
GAGATCG663250.014.047836110-111
GAACTCC88050.013.738425120
Produced by FastQC (version 0.11.5)