FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10741860
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC117650.069.706055120
GAGCACA179450.041.41474120
AGAGCAC236850.033.95316120
AAGAGCA369700.021.375662120
GAAGAGC414100.019.380657118-119
GGAAGAG486800.016.54697120
CGGAAGA576600.014.153902120
TCGGAAG571550.014.134494118-119
GATCGGA684800.012.498424120
ATCGGAA673350.012.140349118-119
AGATCGG761100.011.027695120
TAGATCG163050.09.9211445120
CTAGATC47100.09.712688114-115
GGTGTGT34650.09.5377121
GGTGTAG66450.09.1615071
CACGTCT40400.08.861457120
GGTGTAC51500.08.7813231
CAGATCG349750.08.719526120
GAGATCG323950.08.380789118-119
CCTAGAT19801.8189894E-128.370812120
Produced by FastQC (version 0.11.5)