FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17481693
Sequences flagged as poor quality0
Sequence length50-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC275950.0122.88958120
GAGCACA471100.067.5783120
AGAGCAC642400.051.672604120
AAGAGCA942400.034.91342118-119
GAAGAGC1062400.030.841139116-117
GGAAGAG1286350.025.310452116-117
CGGAAGA1468400.022.362011114-115
TCGGAAG1528750.021.263266114-115
CACACGT66950.020.57069120
CACGTCT70850.020.237196120
ATCGGAA1777850.018.640287112-113
ACACGTC70700.018.145573118-119
GATCGGA1831000.017.916302112-113
AGATCGG2000200.016.65043110-111
GCACACG87550.016.592474120
ACGTCTG88550.015.872462120
TAGATCG554300.015.452918112-113
GAGATCG788700.014.005793110-111
CTAGATC105250.013.847425110-111
CAGATCG664700.013.240935110-111
Produced by FastQC (version 0.11.5)