FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17468693
Sequences flagged as poor quality0
Sequence length50-126
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC282050.0154.79816120
GAGCACA487100.086.0646120
AGAGCAC669800.064.04831118-119
AAGAGCA997150.042.161613118-119
GAAGAGC1121150.038.243748116-117
GGAAGAG1317250.031.85179116-117
CGGAAGA1515450.028.337933114-115
TCGGAAG1603050.026.130829114-115
CACGTCT72300.025.142317120
CACACGT59950.024.672232118-119
ATCGGAA1853550.023.271414112-113
ACACGTC74150.022.687824120
GATCGGA1894000.022.208029112-113
AGATCGG2046700.021.141182110-111
TAGATCG564450.019.194529110-111
GAGATCG644700.018.153885110-111
CAGATCG649450.018.038736112-113
ACGTCTG96000.017.759203120
GCACACG73150.017.595768120
CCCCCCT1750.001798653616.7511311
Produced by FastQC (version 0.11.5)