FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13380382
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC130950.060.80878120
GAGCACA188600.040.6699120
AGAGCAC252000.032.406483120
AAGAGCA392600.021.594707120
GAAGAGC450800.019.244114120
GGAAGAG569950.015.131853118-119
TCGGAAG668250.013.239014120
CGGAAGA682550.012.989598120
GATCGGA790650.012.219149120
ATCGGAA784500.011.909575120
AGATCGG874100.011.096249120
TAGATCG209900.010.272034118-119
TTAGATC95900.09.417534120
CTAGATC54500.09.102602120
CACACGT57950.08.999696120
GAGATCG291950.08.801168120
AAGATCG412500.08.588131120
CAGATCG283800.07.4067597118-119
TAAGATC160550.07.1306586120
CACGTCT60300.06.90865118-119
Produced by FastQC (version 0.11.5)