FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11141906
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC129500.072.20791120
GAGCACA204050.047.108723120
AGAGCAC272600.035.852997120
AAGAGCA422950.023.17973118-119
GAAGAGC476500.020.490276118-119
GGAAGAG574250.017.09017116-117
TCGGAAG692450.014.763465120
CGGAAGA686900.014.540956120
ATCGGAA799900.012.746955118-119
GATCGGA806950.012.348795118-119
AGATCGG896800.011.373493116-117
CTAGATC52450.011.254571120
TAGATCG217200.010.346291114-115
CACGTCT43750.09.966142120
CACACGT36150.09.556469118-119
TTAGATC93200.09.500745114-115
GAGATCG342950.09.251857118-119
AAGATCG319850.09.06002120
ACACGTC47000.08.706101120
CAGATCG281250.08.252521116-117
Produced by FastQC (version 0.11.5)