FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14526544
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC133800.057.467575120
GAGCACA212700.038.243336120
AGAGCAC270000.029.797474120
AAGAGCA433350.020.033028120
GAAGAGC475150.017.909277120
GGAAGAG590850.015.231122120
TCGGAAG657700.014.272865120
CGGAAGA671950.013.582122120
GATCGGA781150.013.010531120
AGATCGG871500.012.099567120
ATCGGAA783800.012.09832120
TAGATCG255300.010.84172118-119
CTAGATC63300.010.1794615118-119
TTAGATC108750.08.522874118-119
GAGATCG342300.08.286681120
AAGATCG314550.08.047224120
CAGATCG336450.08.014898120
GCCTAGA11952.7817352E-57.45097120
GCTAGAT27000.07.4065185116-117
CCTAGAT25600.06.943714114-115
Produced by FastQC (version 0.11.5)