FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831598

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831598
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13757764
Sequences flagged as poor quality0
Sequence length54-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGTG197150.08.400839120
GAGTGTA353100.08.259408120
AGAGTGT382150.08.134039120
GATCTCG239150.08.054619120
TAGATCT240750.08.05094120
AGATCTC242050.07.933325120
AGTGTAG328650.07.9061418120
TCTCGGT204450.07.74867120
TGGTCGC142150.07.683071120
AAGAGTG431850.07.670373120
GTGTAGA327950.07.3740788120
ATCTCGG224400.07.246976120
GTGGTCG151350.07.0971003120
TGTAGAT368950.07.0913563120
TGTAGGG656900.07.061396120
GTAGGGA624250.07.036741118-119
TAGGGAA609900.06.995541120
GTAGATC329700.06.95272120
GGTGGTC175500.06.87273120
GCGTCGG70550.06.7195535120
Produced by FastQC (version 0.11.5)