FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13757764
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC103750.062.043022120
GAGCACA154000.040.52812120
AGAGCAC208450.031.78837120
AAGAGCA345550.019.486525120
GAAGAGC397750.016.997149118-119
GGAAGAG519050.013.54431120
TCGGAAG551200.012.983289120
CGGAAGA623050.011.749408120
GATCGGA624400.011.724004120
ATCGGAA648650.011.139762120
AGATCGG675750.010.657898118-119
TAGATCG168300.09.974208120
CTAGATC43000.09.606481118-119
AAGATCG307900.08.3009205120
CCTAGAT18700.08.099357120
TTAGATC79500.07.932884114-115
GAGATCG260850.07.7970357116-117
CAGATCG223600.06.5760484120
TAAGATC118600.06.301215116-117
TGAGATC118550.06.228237120
Produced by FastQC (version 0.11.5)