FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831602

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831602
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13662321
Sequences flagged as poor quality0
Sequence length64-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA807500.09.264894120
CGTATCA298700.08.897848120
GTGGTCG543750.08.848947120
GGTCGCC494400.08.761435120
TGGTCGC501500.08.637394120
GGTGGTC589550.08.588915120
CCGTATC375200.08.474801120
GTATCAT315700.08.418712120
CGCCGTA446300.07.9043646120
GCCGTAT405250.07.8315763120
TCGGTGG677650.07.7467422120
CTCGGTG742600.07.691278120
CGGTGGT655750.07.557153118-119
ATCTCGG846350.07.5423756120
TCTCGGT775250.07.4602222120
TCATTAA274850.07.421636120
TCGCCGT522050.07.3550286120
GTCGCCG535150.07.2758822120
CATTAAA276300.07.208978120
GATCTCG912900.07.071403120