FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15946324
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC184450.081.76386120
GAGCACA298700.048.32642120
AGAGCAC397850.037.192554118-119
AAGAGCA609000.025.213945120
GAAGAGC687500.022.243017120
GGAAGAG838750.018.362041118-119
CGGAAGA982900.015.865422120
TCGGAAG987950.015.542825116-117
ATCGGAA1163050.013.317543118-119
GATCGGA1190300.013.118707120
AGATCGG1301550.011.851852116-117
TAGATCG281700.011.196118120
CTAGATC70250.09.84936112-113
AAGATCG498600.08.9882765120
TTAGATC109250.08.840959112-113
GAGATCG514150.08.819019118-119
CAGATCG466450.08.237594112-113
ATAGATC143700.07.087955110-111
TAAGATC192450.07.00805118-119
TGAGATC216100.06.9236135120
Produced by FastQC (version 0.11.5)