FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13398823
Sequences flagged as poor quality0
Sequence length53-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA2110450.016.078548120
GCGTCGG71400.013.108074120
TCTCGGG27100.011.73327120
TCATTAA752950.010.159131120
CATTAAA717550.010.133581120
ATCATTA847250.09.963112120
CGTATCA997250.09.595535120
TATCATT980850.09.223829120
GCCGTAT1182350.09.163981120
CGCCGTA1260400.09.082021120
GTATCAT976100.09.072032120
CCGTATC1106100.09.036352120
ATTAAAA772700.08.7192955120
TTAAAAA745600.08.416632120
CATGGTA86900.08.3545271
GTCGCCG1451600.08.307333120
TCGCCGT1366000.08.278911120
GGTCGCC1533050.08.222091120
CATGGTG93950.08.1746551
TGGTCGC1632550.07.8495955120
Produced by FastQC (version 0.11.5)