FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13696871
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC152150.066.05193120
GAGCACA234050.044.165516120
AGAGCAC311300.033.687866120
AAGAGCA471650.022.857409120
GAAGAGC549850.019.543718118-119
GGAAGAG684700.015.856137118-119
TCGGAAG787250.014.116881120
CGGAAGA794850.013.801278120
ATCGGAA922050.012.895277120
GATCGGA936400.012.372114118-119
AGATCGG1049450.011.286301120
CTAGATC65150.010.284051118-119
TAGATCG264900.010.272848120
TTAGATC106500.010.049189120
CACGTCT54050.09.538239120
ACACGTC55950.09.330968120
AAGATCG421450.08.996361120
CACACGT52100.08.802232118-119
GAGATCG373800.08.589395120
TCTAGAT30850.08.249853120
Produced by FastQC (version 0.11.5)