FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14236828
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC160000.072.06028120
GAGCACA251650.043.230446120
AGAGCAC322000.035.06959120
AAGAGCA491450.023.25358120
GAAGAGC564800.020.24625118-119
GGAAGAG667700.017.480883120
TCGGAAG789600.015.099769120
CGGAAGA775400.014.900314118-119
ATCGGAA915200.013.694067120
GATCGGA920700.013.193016118-119
AGATCGG1026100.011.818025118-119
TAGATCG233400.011.587337120
CTAGATC59050.010.445984116-117
TTAGATC81550.09.600323116-117
GAGATCG462650.09.157043116-117
AAGATCG323500.08.360389116-117
CAGATCG370200.08.212025118-119
CCTAGAT25200.08.069838112-113
AGAGATC144850.07.768147116-117
ATAGATC116650.07.3216677118-119
Produced by FastQC (version 0.11.5)