FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15316252
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC189450.081.99461120
GAGCACA304500.050.942596120
AGAGCAC405400.038.344837118-119
AAGAGCA616400.025.160002118-119
GAAGAGC690550.023.21241120
GGAAGAG830750.018.747028118-119
CGGAAGA973850.016.96103120
TCGGAAG991050.015.729353116-117
ATCGGAA1170050.013.696076118-119
GATCGGA1184750.013.22523114-115
AGATCGG1296350.012.308343116-117
TAGATCG324650.011.24385120
CTAGATC85800.010.216117118-119
CACGTCT54050.09.840561120
GAGATCG487200.09.70418110-111
TTAGATC124700.09.654189114-115
AAGATCG411500.09.4019375120
ACACGTC56100.09.09134120
CACACGT49000.09.0704120
CAGATCG475600.08.946896112-113
Produced by FastQC (version 0.11.5)