FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13312871
Sequences flagged as poor quality0
Sequence length50-126
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC213450.0139.54552120
GAGCACA353850.084.332306120
AGAGCAC481100.062.00188118-119
AAGAGCA710500.041.658165118-119
GAAGAGC789400.037.53072116-117
CCGATCT79050.032.7595144
TCCGATC82950.031.5138023
GGAAGAG962300.030.541685116-117
CGATCTA26850.028.9345175
CGGAAGA1130950.026.270617114-115
CACGTCT64550.025.730253120
TCGGAAG1186450.024.870205114-115
CTTCCGA117150.023.4964391
CACACGT61550.022.96284118-119
ACACGTC69200.022.57073120
GCACACG73200.021.788145120
ATCGGAA1383950.021.778616112-113
GATCGGA1410550.021.059875112-113
AGATCGG1524400.019.743187110-111
ACGTCTG78000.019.037176120
Produced by FastQC (version 0.11.5)